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Human Gene Therapy
Photochemical Transfection: A New Technology for Light-Induced, Site-Directed Gene Delivery
To cite this article:
Anders Høgset, Lina Prasmickaite, Torunn E. Tjelle, Kristian Berg.
Human Gene Therapy.
April 2000,
11(6): 869-880.
doi:10.1089/10430340050015482.
Anders Høgset Department of Biophysics, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N-0310 Oslo, Norway. Lina Prasmickaite Department of Biophysics, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N-0310 Oslo, Norway. Department of Biochemistry and Biophysics, University of Vilnius, Ciurlionio 21, 2009 Vilnius, Lithuania. Torunn E. Tjelle Department of Biophysics, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N-0310 Oslo, Norway. Kristian Berg Department of Biophysics, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N-0310 Oslo, Norway. The development of methods for specific delivery of therapeutic genes into target tissues is an important issue for the further progress of in vivo gene therapy. In this article we report on a novel technology, named photochemical transfection, to use light to direct a precise delivery of therapeutic genes to a desired location. The technology makes use of photosensitizing compounds that localize mainly in the membranes of endosomes and lysosomes. On illumination these membrane structures will be destroyed, releasing endocytosed DNA into the cell cytosol. Using a green fluorescent protein gene as a model we show that illumination of photosensitizer-treated cells induces a substantial increase in the efficiency of transfection by DNA–poly-L-lysine complexes. Thus, in a human melanoma cell line the light treatment can increase the transfection efficiency more than 20-fold, reaching transfection levels of about 50% of the surviving cells. In this article various parameters of importance for the use of this technology are examined, and the potential use of the technology in gene therapy is discussed.  This paper was cited by:A Photo-Activated Targeting Chemotherapy Using Glutathione Sensitive Camptothecin-Loaded Polymeric Micelles Horacio Cabral, Masataka Nakanishi, Michiaki Kumagai, Woo-Dong Jang, Nobuhiro Nishiyama, Kazunori Kataoka Pharmaceutical Research. Feb 2009, Vol. 26, No. 1: 82-92 CrossRef Evaluation of Various Polyethylenimine Formulations for Light-Controlled Gene Silencing using Small Interfering RNA Molecules Sigurd Boe, Ane S. Longva, Eivind Hovig Oligonucleotides. Jun 2008, Vol. 18, No. 2: 123-132 Abstract | Full Text PDF | Reprints & PermissionsThe concept of molecular machinery is useful for design of stimuli-responsive gene delivery systems in the mammalian cell Takeshi Nagasaki, Seiji Shinkai Journal of Inclusion Phenomena and Macrocyclic Chemistry. Aug 2007, Vol. 58, No. 3-4: 205-219 CrossRef Photochemically Induced Gene Silencing Using Small Interfering RNA Molecules in Combination with Lipid Carriers S. Bøe, A.S. Longva, E. Hovig Oligonucleotides. 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Jun 2006, Vol. 16, No. 2: 145-157 Abstract | Full Text PDF | Reprints & PermissionsPhotochemically enhanced transduction of polymer-complexed adenovirus targeted to the epidermal growth factor receptor Anette Bonsted, Birgit Øvstebø Engesæter, Anders Høgset, Gunhild M. Mælandsmo, Lina Prasmickaite, Christine D'Oliveira, Wim E. Hennink, Jan Hein van Steenis, Kristian Berg The Journal of Gene Medicine. Apr 2006, Vol. 8, No. 3: 286-297 CrossRef Photochemical Internalization of Transgenes Controlled by the Heat-shock Protein 70 Promoter Lina Prasmickaite, Marit Hellum, Olav Kaalhus, Anders Høgset, Ernst Wagner, Kristian Berg Photochemistry and Photobiology. Feb 2006, Vol. 82, No. 3: 809 CrossRef Photochemically enhanced adenoviral transduction in a multicellular environment Anette Bonsted, Birgit Øvstebø Engesæter, Anders Høgset, Kristian Berg Photochemical & Photobiological Sciences. Feb 2006, Vol. 5, No. 4: 411 CrossRef Light-induced gene transfer from packaged DNA enveloped in a dendrimeric photosensitizer Nobuhiro Nishiyama, Aya Iriyama, Woo-Dong Jang, Kanjiro Miyata, Keiji Itaka, Yuji Inoue, Hidenori Takahashi, Yasuo Yanagi, Yasuhiro Tamaki, Hiroyuki Koyama, Kazunori Kataoka Nature Materials. Jan 2006, Vol. 4, No. 12: 934-941 CrossRef Intelligent polymers as nonviral vectors S Dinçer, M Türk, E Pişkin Gene Therapy. Nov 2005, Vol. 12: S139-S145 CrossRef Porphyrin-related photosensitizers for cancer imaging and therapeutic applications K. BERG, P. K. SELBO, A. WEYERGANG, A. DIETZE, L. PRASMICKAITE, A. BONSTED, B. O. ENGESAETER, E. ANGELL-PETERSEN, T. WARLOE, N. FRANDSEN, A. HOGSET Journal of Microscopy. Jun 2005, Vol. 218, No. 2: 133-147 CrossRef PCI-enhanced adenoviral transduction employs the known uptake mechanism of adenoviral particles Birgit Ø Engesæter, Anette Bonsted, Kristian Berg, Anders Høgset, Olav Engebråten, Øystein Fodstad, David T Curiel, Gunhild M Mælandsmo Cancer Gene Therapy. Feb 2005 CrossRef Targeted polymers for gene delivery Jeffery A Hughes, Gururaj A Rao Expert Opinion on Drug Delivery. Feb 2005, Vol. 2, No. 1: 145-157 CrossRef Light-directed gene delivery by photochemical internalisation Lina Prasmickaite, Anders Høgset, Birgit Ø Engesæter, Anette Bonsted, Kristian Berg Expert Opinion on Biological Therapy. Oct 2004, Vol. 4, No. 9: 1403-1412 CrossRef Photochemical Internalization Enhances the Cytotoxic Effect of the Protein Toxin Gelonin and Transgene Expression in Sarcoma Cells¶ A. Dietze, A. Bonsted, A. Høgset, K. Berg Photochemistry and Photobiology. Feb 2003, Vol. 78, No. 3: 283 CrossRef Photo-enhancement of Transfection Efficiency with a Novel Azobenzene-based Cationic Lipid Takeshi Nagasaki, Katsutoshi Wada, Seizo Tamagaki Chemistry Letters. Feb 2003, Vol. 32, No. 1: 88-89 CrossRef Photochemical disruption of endocytic vesicles before delivery of drugs: a new strategy for cancer therapy L Prasmickaite, A Høgset, P K Selbo, BØ Engesæter, M Hellum, K Berg British Journal of Cancer. Mar 2002, Vol. 86, No. 4: 652-657 CrossRef In vivo documentation of photochemical internalization, a novel approach to site specific cancer therapy P  l Kristian Selbo, Gowsala Sivam, ystein Fodstad, Kirsten Sandvig, Kristian Berg International Journal of Cancer. Jul 2001, Vol. 92, No. 5: 761-766 CrossRef Gene medicine: A new field of molecular medicine Chong-Kook Kim, Kh. H Haider, Soo-Jeong Lim Archives of Pharmacal Research. Mar 2001, Vol. 24, No. 1: 1-15 CrossRef Evaluation of Different Photosensitizers for Use in Photochemical Gene Transfection¶ Lina Prasmickaite, Anders Høgset, Kristian Berg Photochemistry and Photobiology. Feb 2001, Vol. 73, No. 4: 388 CrossRef Role of endosomes in gene transfection mediated by photochemical internalisation (PCI) Lina Prasmickaite, Anders H gset, Torunn E. Tjelle, Vibeke M. Olsen, Kristian Berg The Journal of Gene Medicine. Dec 2000, Vol. 2, No. 6: 477-488 CrossRef Photochemical internalisation increases the cytotoxic effect of the immunotoxin MOC31-gelonin Pål Kristian Selbo, Gowsala Sivam, Oystein Fodstad, Kirsten Sandvig, Kristian Berg International Journal of Cancer. Oct 2000, Vol. 87, No. 6: 853-859 CrossRef |
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