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Tissue Engineering
Human Adipose-Derived Adult Stem Cells Produce Osteoid in Vivo
To cite this article:
Kevin C. Hicok, Tracey V. du Laney, Yang Sheng Zhou, Yuan-Di C. Halvorsen, Daron C. Hitt, Lyndon F. Cooper, Jeffrey M. Gimble.
Tissue Engineering.
March 2004,
10(3-4): 371-380.
doi:10.1089/107632704323061735.
Kevin C. Hicok Artecel Sciences, Durham, North Carolina Tracey V. du Laney Artecel Sciences, Durham, North Carolina Yang Sheng Zhou Department of Prosthodontics, School of Stomatology, Peking University, Beijing, China Yuan-Di C. Halvorsen Sciences, Durham, North Carolina Daron C. Hitt Department of Plastic Surgery, UTHSC, San Antonio, Texas Lyndon F. Cooper Department of Prosthodontics, University of North Carolina Jeffrey M. Gimble Artecel Sciences, Durham, North Carolina Adult subcutaneous fat tissue is an abundant source of multipotent cells. Previous studies from our laboratory have shown that, in vitro, adipose-derived adult stem (ADAS) cells express bone marker proteins including alkaline phosphatase, type I collagen, osteopontin, and osteocalcin and produce a mineralized matrix as shown by alizarin red staining. In the current study, the ADAS cell ability to form osteoid in vivo was determined. ADAS cells were isolated from liposuction waste of three individual donors and expanded in vitro before implantation. Equal numbers of cells (3 × 106) were loaded onto either hydroxyapatite/tricalcium phosphate (HA-TCP) cubes or the collagen/HA-TCP composite matrix, Collagraft, and then implanted subcutaneously into SCID mice. After 6 weeks, implants were removed, fixed, and demineralized and sectioned for hematoxylin and eosin staining. Osteoid formation was observed in 80% of HA-TCP implants loaded with ADAS cells. Only 20% of Collagraft implants were positive for the presence of osteoid matrix. Whereas 100% of HA-TCP implants loaded with hFOB 1.19 cells formed osteoid, Collagraft loaded with hFOB 1.19 cells displayed a high degree of adipose tissue within the matrix. Immunostaining of serial sections for human nuclear antigen demonstrated that the osteoid contained human cells. Osteoid formation was not observed in control HA-TCP or Collagraft matrices implanted without cells. 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