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Cloning and Stem Cells
In Vitro Osteogenic Differentiation of Human ES Cells
To cite this article:
Virginie Sottile, Alison Thomson, Jim McWhir. Cloning and Stem Cells. August 2003, 5(2): 149-155. doi:10.1089/153623003322234759.
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Virginie Sottile
Department of Gene Expression and Development, Roslin Institute, Roslin, Midlothian, United Kingdom.
Alison Thomson
Department of Gene Expression and Development, Roslin Institute, Roslin, Midlothian, United Kingdom.
Jim McWhir
Department of Gene Expression and Development, Roslin Institute, Roslin, Midlothian, United Kingdom.

Since their isolation in 1998, human embryonic stem (hES) cells have been shown to be capable of adopting various cell fates in vitro. Here, we present in vitro data demonstrating the directed commitment of human embryonic stem cells to the osteogenic lineage. Human ES cells are shown to respond to factors that promote osteogenesis, leading to activation of the osteogenic markers osteocalcin, parathyroid hormone receptor, bone sialoprotein, osteopontin, cbfa1, and collagen 1. Moreover, the mineralized nodules obtained are composed of hydroxyapatite, further establishing the similarity of osteoblasts in culture to bone. These results show that osteoblasts can be derived from human ES cultures in vitro and provide the basis for comparison of adult and embryonic-derived osteogenesis, and for an investigation of potential applications for hES cells in orthopaedic tissue repair.

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