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Journal of Computational Biology
Validation of S. Pombe Sequence Assembly by Microarray Hybridization
To cite this article:
Joseph West, John Healy, Michael Wigler, William Casey, Bud Mishra.
Journal of Computational Biology.
January/February 2006,
13(1): 1-20.
doi:10.1089/cmb.2006.13.1.
Joseph West Cold Spring Harbor Laboratory, 1 Bungtown Road, P.O. Box 100, Cold Spring Harbor, NY 11724. John Healy Cold Spring Harbor Laboratory, 1 Bungtown Road, P.O. Box 100, Cold Spring Harbor, NY 11724. Michael Wigler Cold Spring Harbor Laboratory, 1 Bungtown Road, P.O. Box 100, Cold Spring Harbor, NY 11724. William Casey Courant Institute of Mathematical Sciences, 251 Mercer Street, New York, NY 10012. Bud Mishra Courant Institute of Mathematical Sciences, 251 Mercer Street, New York, NY 10012. Department of Cell Biology, NYU School of Medicine, New York, NY 10016. We describe a method to make physical maps of genomes using correlative hybridization patterns of probes to random pools of BACs. We derive thereby an estimated distance between probes, and then use this estimated distance to order probes. To test the method, we used BAC libraries from Schizzosaccharomyces pombe. We compared our data to the known sequence assembly, in order to assess accuracy. We demonstrate a small number of significant discrepancies between our method and the map derived by sequence assembly. Some of these discrepancies may arise because genome order within a population is not stable; imposing a linear order on a population may not be biologically meaningful.
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