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DNA and Cell Biology
Dynamic Intracellular Distribution of Eaf2 and Its Potential Involvement in UV-Induced DNA Damage Response
To cite this article:
Fengfeng Zhuang, Philip Yen, Jiangyue Zhao, Manuel Nguyen, Meisheng Jiang, Yi-Hsin Liu.
DNA and Cell Biology.
December 2008,
27(12): 649-656.
doi:10.1089/dna.2008.0733.
Fengfeng Zhuang,1,2 Philip Yen,1,2 Jiangyue Zhao,1,3 Manuel Nguyen,1 Meisheng Jiang,4 and Yi-Hsin Liu1,2 1Department of Ophthalmology, Keck School of Medicine, University of Southern California, Los Angeles, California. 2Graduate Program in Craniofacial Biology, Center for Craniofacial Molecular Biology, School of Dentistry, University of Southern California, Los Angeles, California. 3Department of Ophthalmology, China Medical University, Shenyang, China. 4Department of Molecular and Medical Pharmacology, UCLA School of Medicine, Los Angeles, California. Address reprint requests to: Yi-Hsin Liu, Ph.D. Department of Ophthalmology Keck School of Medicine University of Southern California1355 San Pablo St., DVRC314 Los Angeles, CA 90033 E-mail: 14 1 2008 Received for publication January 14, 2008 5 8 2008 received in revised form August 5, 2008 8 8 2008 accepted August 8, 2008 Eaf2 encodes a tumor suppressor that plays multiple functions in transcriptional activation, apoptosis, and embryonic development. In this study, we utilized GFP-EAF2 fusion protein to describe the dynamic subcellular movement of Eaf2. GFP-EAF2 is preferentially localized to the nucleus, and in the presence of ELL, it accumulates in nuclear speckles. However, Eaf2 is an unstable nuclear protein whose stability is affected by serum. Further, we provided first evidence that nuclear distribution of Eaf2 is responsive to DNA damage. Following UV irradiation, Eaf2 is relocalized to the nucleolus, suggesting a possible functional involvement of Eaf2 in DNA damage response. |
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