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Human Gene Therapy
Wnt Inhibitory Factor-1 Gene Transfer Inhibits Melanoma Cell Growth
To cite this article:
Yu-Ching Lin, Liang You, Zhidong Xu, Biao He, Cheng-Ta Yang, Jan-Kan Chen, Iwao Mikami, Geneviève Clément, Yihui Shi, Kristopher Kuchenbecker, Junichi Okamoto, Mohammed Kashani-Sabet, David M. Jablons.
Human Gene Therapy.
April 2007,
18(4): 379-386.
doi:10.1089/hum.2006.005.
Yu-Ching Lin Thoracic Oncology Laboratory, Department of Surgery, University of California, San Francisco, San Francisco, CA 94115. Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Chang Gung Memorial Hospital, Chiayi, Taiwan 613. Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan 333. Liang You Thoracic Oncology Laboratory, Department of Surgery, University of California, San Francisco, San Francisco, CA 94115. Zhidong Xu Thoracic Oncology Laboratory, Department of Surgery, University of California, San Francisco, San Francisco, CA 94115. Biao He Thoracic Oncology Laboratory, Department of Surgery, University of California, San Francisco, San Francisco, CA 94115. Cheng-Ta Yang Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Chang Gung Memorial Hospital, Chiayi, Taiwan 613. Department of Respiratory Care, College of Medicine, Chang Gung University, Taoyuan, Taiwan 333. Jan-Kan Chen Department of Physiology and Pharmacology, College of Medicine, Chang Gung University, Taoyuan, Taiwan 333. Iwao Mikami Thoracic Oncology Laboratory, Department of Surgery, University of California, San Francisco, San Francisco, CA 94115. Geneviève Clément Thoracic Oncology Laboratory, Department of Surgery, University of California, San Francisco, San Francisco, CA 94115. Yihui Shi Thoracic Oncology Laboratory, Department of Surgery, University of California, San Francisco, San Francisco, CA 94115. Kristopher Kuchenbecker Thoracic Oncology Laboratory, Department of Surgery, University of California, San Francisco, San Francisco, CA 94115. Junichi Okamoto Thoracic Oncology Laboratory, Department of Surgery, University of California, San Francisco, San Francisco, CA 94115. Mohammed Kashani-Sabet Auerback Melanoma Research Laboratory, Melanoma Center and Department of Dermatology, and Cutaneous Oncology Program, Comprehensive Cancer Center, University of California, San Francisco, San Francisco, CA 94115. David M. Jablons Thoracic Oncology Laboratory, Department of Surgery, University of California, San Francisco, San Francisco, CA 94115. Silencing of Wnt antagonists with aberrant activation of Wnt signaling is a common phenomenon in various human cancers. Wnt inhibitory factor-1 (WIF-1) is a secreted antagonist of Wnt signaling and acts through direct binding to Wnt in the extracellular space. In this study, we tried to illuminate the impact of WIF-1 gene expression in melanoma with WIF-1 silencing by in vitro and in vivo studies. We restored the expression of WIF-1 by nonviral gene transfer with a pcDNA3.1 vector. We demonstrated inhibition of melanoma cell growth after WIF-1 restoration in colony formation and proliferation assays in vitro. In addition, the inhibitory effect was related to downregulation of Wnt signaling, which was demonstrated at both the transcriptional and translational levels. Furthermore, by using a xenograft mouse model, we confirmed the effect of WIF-1 expression in suppressing tumor growth by inhibition of Wnt signaling in vivo. Our results suggest the potential for further application of WIF-1 gene therapy in melanoma with WIF-1 silencing.  This paper was cited by:A Wnt Survival Guide: From Flies to Human Disease Andy J Chien, William H Conrad, Randall T Moon Journal of Investigative Dermatology. Aug 2009, Vol. 129, No. 7: 1614-1627 CrossRef G-protein-coupled receptors and melanoma Hwa Jin Lee, Brian Wall, Suzie Chen Pigment Cell & Melanoma Research. Sep 2008, Vol. 21, No. 4: 415-428 CrossRef Nasopharyngeal carcinoma—Review of the molecular mechanisms of tumorigenesis Josephine Chou, Yu-Ching Lin, Jae Kim, Liang You, Zhidong Xu, Biao He, David M. Jablons Head & Neck. Aug 2008, Vol. 30, No. 7: 946-963 CrossRef Wnt signalling and its impact on development and cancer Alexandra Klaus, Walter Birchmeier Nature Reviews Cancer. Jun 2008, Vol. 8, No. 5: 387-398 CrossRef Understanding Signaling Cascades in Melanoma Pablo Lopez-Bergami, Boris Fitchman, Ze’ev Ronai Photochemistry and Photobiology. Apr 2008, Vol. 84, No. 2: 289-306 CrossRef Docking of Fatty Acids into the WIF Domain of the Human Wnt Inhibitory Factor-1 Tomas Malinauskas Lipids. Apr 2008, Vol. 43, No. 3: 227-230 CrossRef |
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