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Human Gene Therapy
In Vivo Culture of Encapsulated Endostatin-Secreting Chinese Hamster Ovary Cells for Systemic Tumor Inhibition
To cite this article:
Ying Zhang, Wei Wang, Yubing Xie, Weiting Yu, Huaining Teng, Xiudong Liu, Xulang Zhang, Xin Guo, Jian Fei, Xiaojun Ma.
Human Gene Therapy.
May 2007,
18(5): 474-481.
doi:10.1089/hum.2006.166.
Ying Zhang Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, People's Republic of China. Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100039, People's Republic of China. Wei Wang Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, People's Republic of China. Yubing Xie Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, People's Republic of China. Weiting Yu Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, People's Republic of China. Huaining Teng Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, People's Republic of China. Xiudong Liu Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, People's Republic of China. Xulang Zhang Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, People's Republic of China. Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100039, People's Republic of China. Xin Guo Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, People's Republic of China. Jian Fei Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, People's Republic of China. Xiaojun Ma Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, People's Republic of China. Microencapsulation of recombinant cells is a novel alternative approach to tumor gene therapy. Therapeutic protein delivery can be sustained for systemic treatment of tumors because the recombinant cells are enclosed in microcapsules and the semipermeable membrane of the microcapsules protects the cells from host immune rejection and reduces the need for frequent injection. In this study, we describe a method to systemically inhibit tumor growth by in vivo culture of antiangiogenic endostatin-secreting Chinese hamster ovary (CHO) cells in microcapsules as small as 200μm in diameter. Peritoneal administration of encapsulated endostatin-CHO cells inhibited melanoma growth to 66.4% and enhanced the survival of treated mice to 80% by 27 days posttreatment. Continuous systemic release of endostatin from microcapsules offers an effective therapeutic strategy to eradicate solid tumors.  This paper was cited by:Effect of surface wettability and charge on protein adsorption onto implantable alginate-chitosan-alginate microcapsule surfaces Hong-Guo Xie, Xiao-Xia Li, Guo-Jun Lv, Wei-Yang Xie, Jing Zhu, Thomas Luxbacher, Ron Ma, Xiao-Jun Ma Journal of Biomedical Materials Research Part A. Feb 2009, Vol. 9999A: NA-NA CrossRef |
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