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Hybridoma
Plant-produced Hepatitis B Core Protein Chimera Carrying Anthrax Protective Antigen Domain-4
To cite this article:
Katarzyna Bandurska, Robert Brodzik, Sergei Spitsin, Thomas Kohl, Carla Portocarrero, Yuri Smirnov, Natalia Pogrebnyak, Agnieszka Sirko, Hilary Koprowski, Maxim Golovkin.
Hybridoma.
August 2008,
27(4): 241-247.
doi:10.1089/hyb.2008.0008.
Katarzyna Bandurska Biotechnology Foundation Laboratories, Thomas Jefferson University, Philadelphia, Pennsylvania. Robert Brodzik Biotechnology Foundation Laboratories, Thomas Jefferson University, Philadelphia, Pennsylvania. Sergei Spitsin Biotechnology Foundation Laboratories, Thomas Jefferson University, Philadelphia, Pennsylvania. Thomas Kohl Biotechnology Foundation Laboratories, Thomas Jefferson University, Philadelphia, Pennsylvania. Carla Portocarrero Biotechnology Foundation Laboratories, Thomas Jefferson University, Philadelphia, Pennsylvania. Yuri Smirnov Biotechnology Foundation Laboratories, Thomas Jefferson University, Philadelphia, Pennsylvania. Natalia Pogrebnyak Biotechnology Foundation Laboratories, Thomas Jefferson University, Philadelphia, Pennsylvania. Agnieszka Sirko Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland. Hilary Koprowski Biotechnology Foundation Laboratories, Thomas Jefferson University, Philadelphia, Pennsylvania. Maxim Golovkin Biotechnology Foundation Laboratories, Thomas Jefferson University, Philadelphia, Pennsylvania. Abstract The hepatitis B core antigen (HBcAg) can generate a strong immune response and is recognized as an effective carrier for foreign epitopes. The domain-4 epitope of the anthrax protective antigen (PA-D4) plays an essential role in generating protective immunity against virulent Bacillus anthracis. Here we report the successful production of a recombinant protein comprised of the antigenic PA-D4 integrated into the c/e1 loop of HBcAg in transgenic low-alkaloid Nicotiana tabacum. Sera of mice injected with the plant-derived purified HB/PA-D4 protein exhibited significant anti-PA- and anti-HBcAg-specific IgG titers; however, formation of virus-like particles (VLP) was not observed. These data support the feasibility of producing complex protein chimeras in plants.
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