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Tissue Engineering
Isolation and Characterization of Postnatal Stem Cells from Human Dental Tissues
To cite this article:
You-Young Jo, Hee-Jung Lee, Sun-Young Kook, Han-Wool Choung, Joo-Young Park, Jong-Hoon Chung, Yun-Hoon Choung, Eun-Suk Kim, Hyeong-Cheol Yang, Pill-Hoon Choung.
Tissue Engineering.
April 2007,
13(4): 767-773.
doi:10.1089/ten.2006.0192.
You-Young Jo, Ph.D.Department of Oral and Maxillofacial Surgery, Tooth Bioengineering National Research Lab, BK21, and Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea. Hee-Jung Lee, M.S.Department of Oral and Maxillofacial Surgery, Tooth Bioengineering National Research Lab, BK21, and Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea. Sun-Young Kook, B.S.Department of Oral and Maxillofacial Surgery, Tooth Bioengineering National Research Lab, BK21, and Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea. Han-Wool Choung, D.D.S.Department of Oral and Maxillofacial Surgery, Tooth Bioengineering National Research Lab, BK21, and Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea. Joo-Young Park, D.D.S.Department of Oral and Maxillofacial Surgery, Tooth Bioengineering National Research Lab, BK21, and Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea. Jong-Hoon Chung, Ph.D.Department of Biosystems & Biomaterials Science and Engineering, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea. Yun-Hoon Choung, D.D.S., M.D., Ph.D.Department of Otolaryngology, Ajou University, School of Medicine, Suwon, Korea. Eun-Suk Kim, D.D.S., M.S.D., Ph.D.Department of Oral and Maxillofacial Surgery, School of Medicine, Choongnam National University, Tae-jun, Korea. Hyeong-Cheol Yang, Ph.D.Department of Dental Biomaterials Science, School of Dentistry, Seoul National University, Seoul, Korea. Pill-Hoon Choung, D.D.S., Ph.D.Department of Oral and Maxillofacial Surgery, Tooth Bioengineering National Research Lab, BK21, and Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea. It was reported that postnatal stem cells are present in adult tissues such as bone marrow, liver, muscle, dental pulp, and periodontal ligament. We isolated postnatal stem cells from human dental tissues such as dental pulp (DPSC), periodontal ligament (PDLSC), periapical follicle (PAFSC), and the surrounding mandibular bone marrow (MBMSC) to ascertain their properties. Immunocytochemistry proved the existence of stem cells in these cell populations using STRO-1 as a stem cell marker. These cells also expressed the mesenchymal stem cell (MSC) markers CD29 and CD44. The isolated cells showed self-renewal capabilities and colony-forming efficiency. Almost all of the dental stem cells showed optimal growth when they were cultured in alpha modification of Eagle's medium (alpha-MEM) supplemented with 10% fetal calf serum (FCS) and 100 μM ascorbic acid. Only the PAFSC showed increased proliferation in 20% FCS and 50 μM ascorbic acid. All of the dental stem cells were capable of differentiating into adipocytes and mineral nodule forming cells. MBMSC, in particular, showed much better mineralization compared to the others. These results indicate that MSCs exist in various tissues of the teeth and can differentiate into osteoblasts, adipocytes, and other kinds of cells with varying efficiency.  This paper was cited by:Human dental pulp stem cells: from biology to clinical applications Riccardo d'Aquino, Alfredo De Rosa, Gregorio Laino, Filippo Caruso, Luigi Guida, Rosario Rullo, Vittorio Checchi, Luigi Laino, Virginia Tirino, Gianpaolo Papaccio Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. Aug 2009, Vol. 312B, No. 5: 408-415 CrossRef Bone Forming Capacity of Mesenchymal Stromal Cells when Cultured in the Presence of Human Platelet Lysate as Substitute for Fetal Bovine Serum Henk-Jan Prins, Henk Rozemuller, Simone Vonk-Griffioen, Vivienne G.M. Verweij, Wouter Dhert, Ineke Slaper-Cortenbach, Anton C.M. Martens Tissue Engineering Part A. , Vol. 0, No. ja Abstract | Full Text PDFEncapsulation of Mesenchymal Stem Cells
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1 Zuo-lin JIN, Yong-kuan ZHANG, Hai-yan SUN, Zhu LIN, Ying-chun BI, Yin-zhong DUAN, Yin DING Acta Pharmacologica Sinica. Oct 2008, Vol. 29, No. 9: 1013-1020 CrossRef Current Approaches and Challenges in Making a Bio-Tooth Jinhua Yu, Junnan Shi, Yan Jin Tissue Engineering Part B: Reviews. Sep 2008, Vol. 14, No. 3: 307-319 Abstract | Full Text PDF | Reprints & PermissionsSomatic stem cells for regenerative dentistry Christian Morsczeck, Gottfried Schmalz, Torsten Eugen Reichert, Florian Völlner, Kerstin Galler, Oliver Driemel Clinical Oral Investigations. Jul 2008, Vol. 12, No. 2: 113-118 CrossRef Stem cells and periodontal regeneration N-H Lin, S Gronthos, PM Bartold Australian Dental Journal. Jul 2008, Vol. 53, No. 2: 108-121 CrossRef Collection, Cryopreservation, and Characterization of Human Dental Pulp–Derived Mesenchymal Stem Cells for Banking and Clinical Use Brandon C. Perry, Dan Zhou, Xiaohua Wu, Feng-Chun Yang, Michael A. Byers, T.-M. Gabriel Chu, J. Jeffrey Hockema, Erik J. Woods, W. Scott Goebel Tissue Engineering Part C: Methods. Jun 2008, Vol. 14, No. 2: 149-156 Abstract | Full Text PDF | Reprints & PermissionsStem cells and tooth tissue engineering Amanda H.-H. Yen, Paul T. Sharpe Cell and Tissue Research. Feb 2008, Vol. 331, No. 1: 359-372 CrossRef |
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